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H8142
Sigma
Hybridoma Enhancing Supplement from murine macrophage-like cell line
liquid, sterile-filtered, hybridoma tested
| Synonym: | Conditioned Medium from murine macrophage-like cell line |
Description
| Analysis Note | Cell line viral testing: Murine Antibody Production (map) testing for Sendai, MHV, PVM, Reo3, GDVII, Ectomelia, MVM, Polyoma, LDMV, M. Ad, EDIM, MCMV, LCM, Hantaan. BVD virus by fluorescent antibody stain |
| Application | Use at a concentration of 10% in complete medium containing FBS (10%) or CPRS-3. Optimal conditions must be determined for specific applications. |
| Caution | Avoid repeated freezing and thawing. If entire contents are not used, store frozen in working aliquots. Precipitation may occur when stored. This is not detrimental to the product and may be reversed when warmed to 37 °C. |
| Other Notes | Conditioned medium from a proprietary murine macrophage-like cell line grown in IMDM with 2% FBS. |
Properties
| sterility | sterile-filtered |
| form | liquid |
| total impurities | Endotoxin, tested |
| Mycoplasma, tested | |
| suitability | hybridoma tested |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator (EU), half-mask respirator (US), multi-purpose combination respirator cartridge (US) |
| WGK Germany | 3 |
